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KMID : 1147820220110020109
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Journal of Naturopathy
2022 Volume.11 No. 2 p.109 ~ p.115
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Physiological Activities of wild Conyza canadensis L. Extracts
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Lee Hee-Jea
Song Hyun-Sook
Lee Geo-Lyong
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Abstract
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Background: Studies on the existence and activation of antioxidants in the wild Erigeron canadensis are still incomplete.
Purposes: The activity of antioxidant substances was studied by extracting E. canadensis with ethyl alcohol.
Methods: After washing the wild turfgrass, extraction with ethyl alcohol was used to investigate the activity of antioxidant substances using various analytical instruments.
Results: The highest yield ratio of the extract was 49.3%, and it varied according to temperature and ethyl alcohol ratio. The 50:50 of water to ethyl alcohol at 180°C was the highest yield. 100% survival rate was in the untreated group and 98-100% in the experimental group at 50 ppm or more of the extract. There was no cytotoxicity at almost all concentrations. The extract of 25 ppm was suppressed by 42% in the test group. The extract of 50 ppm reduced the free fatty acid content by 15%. Cell viability was 20% at the extract concentration of 50 ppm and 24% at 100 ppm. At an extract of 500 ppm, the free oxygen scavenging ability using DPPH and ABTs was 90-98%. When the changes in the free radical scavenging activity of DPPH and ABTs were observed in three dimensions, the antioxidant activity tended to increase at 85o C as the temperature increased. However, at 85~130°C, it showed a rather decreasing tendency as the temperature increased.
Conclusions: There were antioxidants in the ethyl alcohol extract of the wild grass, there was almost no cytotoxicity and suppressed NO production, and the scavenging function of free oxygen was also high. These results provide primary data for the various natural healing uses of the extracts of the turfgrass.
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KEYWORD
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E. canadensis, Ethyl alcohol extract, Antioxidant Cell Cytotoxicity
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